Scientists Implant Biofuel Cell in Living Snail

By Maria Panagiotidi

Researchers led by Evgeny Katz, the Milton Kerker Chaired Professor of Colloid Science at Clarkson University, have implanted a biofuel cell in a living snail. Their findings were published in the Journal of The American Chemical Society.

Researchers led by Evgeny Katz, the Milton Kerker Chaired Professor of Colloid Science at Clarkson University, have implanted a biofuel cell in a living snail. This is the first incidence of an implanted biofuel cell continuously operating in a snail and producing electrical power over a long period of time using the snail’s physiologically produced glucose as a fuel. (Credit: Image courtesy of Clarkson University)

The “implanted battery” can generate electrical power for several months driven by glucose, which is produced by the snail.

This is the first reported incident of an implanted biofuel cell operating in a snail and producing electrical power over a long period of time using as fuel the glucose that is physiologically generated by its host.

Implantable biofuel cells have been suggested as sustainable micropower sources operating in living organisms, but such systems are still very challenging to design. In the future, implanted fuel cells that are driven by glucose generated by their host could power medical devices in humans or environmental sensors in animals.

Evgeny Katz and his colleagues made the electrodes of their fuel cell out of densely packed carbon nanotubes, and attached glucose-oxidizing and oxygen-reducing enzymes to them. The authors then implanted the electrodes into a snail (Neohelix albolabris). After decreasing the rate of current extraction to match the snail’s slow glucose transport and metabolism, they got continuous electrical output for an hour. The amount of electricity produced was far below that of just one AAA battery, but the group of scientists aim to increase it in future experiments. The fuel cell remained functional in the snail for several months during which the animal was allowed to roam freely and live an almost normal life.

The aim of this research is creating insect cyborgs, an idea that has been funded by the U.S. Department of Defense.

 

Reference

Lenka Halámková, Jan Halámek, Vera Bocharova, Alon Szczupak, Lital Alfonta, Evgeny Katz. Implanted Biofuel Cell Operating in a Living SnailJournal of the American Chemical Society, 2012; : 120308155036002 DOI:10.1021/ja211714w

You can find the article here:  http://pubs.acs.org/doi/abs/10.1021/ja211714w 

 

 

DNA Extraction from Strawberries by Michaela

Another experiment we did in Einstein’s Kitchen was extracting the DNA from strawberries, and wowed kids and adults alike!

Please do not eat anything (apart from the strawberries) in this experiment and if you’re doing this with small children please be extra vigilant to make sure they don’t put anything in their mouths.

DNA. It’s the blueprint for life. DNA spells out the instructions to all living things to tell them how to become what they are, and how their cells should work. Discovered back in 1869 as a part of the cell’s nucleus, it wasn’t until the 1950s that some very clever people realised that DNA was in fact the genetic material – the thing that carried genes and was responsible for heredity – the passage of characteristics from one generation to the next – why you look like your parents!  Since then there’s been a surge in research studying how traits are passed on and how the message in genes leads to a cell working the way it does.

DNA stands for deoxyribonucleic acid – quite a boring name that just describes the chemical structure (shown below). It’s basically a big string of units, where each unit has a different letter, A, T, G or C that the cell reads. These strands form the infamous double helix structure.

As scientists we’re interested in extracting, or getting at, DNA for a number of reasons, for example, to read the sequence of it to compare it from organism to organism, diagnosing genetic diseases, and to use it as a tool to study processes in the cell. DNA can be used from blood and other samples found at crime scenes to identify who could have been there, and the same principle is behind paternity tests.

 

To get at the DNA we scientists carry out a technique not too dissimilar to what we carried out at the festival, and that you can try yourself at home!

So here’s what you’ll need:

  • ·         A cup and a squashing instrument (like a fork)
  • ·         One strawberry
  • ·         10mL of DNA extraction buffer (add 2 tsp of salt to 50mL washing up liquid and then make that up to one litre with water).
  • ·         Cheesecloth
  • ·         A funnel
  • ·         A shot glass
  • ·         A thin stick
  • ·         10mL ice cold alcohol (we used 100% ethanol, but white rum works!). Just stick it in the freezer for a couple hours to cool it.

What you need to do is actually really easy but I’ve included an explanation for each step, so don’t be put off by the length of this:

1.          DNA as mentioned before is found inside the cell’s nucleus, so the first thing you’ll need to do is break open the cells. Simply place your strawberry in the cup and start mushing!

2.          Cells are actually really tiny things, so though after a minute or two of mushing your strawberry won’t be looking much like a strawberry, chances are that a lot of the cells are still intact! So to really help break it up add 10mL of the extraction buffer (about 2 teaspoons) and carry on stirring. Cells are held together in a sack of fatty acids called the membrane. Washing up liquid is a detergent, and in the same way as it cleans off grease it will dissolve the membrane meaning that the cell bursts open to release everything.

 

3.          This mix will contain everything inside the cell including the DNA dissolved in the liquid, as well as a lot of big bits that you’ll want to get rid of, so the next thing to do is filter the liquid away from all the big bits. To do this simply place your cheesecloth inside the funnel and pour your mushin. Give it a bit of a stir so that the liquid passes through to be collected in the shot glass.

4.          Now the next step is almost like magic, but it’s not actually. We can make the DNA appear out of the liquid! That’s because the DNA is dissolved in the liquid but we can make it so it no longer ‘likes’ water and so will turn to a solid – this is called precipitation. Slowly add 10mL ice-cold ethanol and be careful not to disturb the mush (or spray strawberry all over your kitchen). You should see a fluffy white solid forming at the junction between mush and alcohol. This is a little tricky, but bear with me: remember in your extraction buffer you added salt – salts sticks to the negative charges on DNA and neutralises them. Now in chemistry there’s a bit of a rule where things that are the same will dissolve each other but things that aren’t the same won’t dissolve in each other. Alcohol has a charge so by making the DNA unlike the alcohol by neutralising its chargesit means that it won’t dissolve any longer – it will come out of solution as a solid.

 

5.         Get your stick and fish out the white stuff – that is DNA!

 

Every living thing uses this very same chemical, and if you extracted DNA from yourself or a worm it would look exactly the same as strawberry DNA. Strawberries are great for using in this experiment because they contain so much DNA! We have two copies of each of our chromosomes, but strawberries have 8! This has mostly come about through selective breeding by farmers to get bigger strawberries! You might be wondering why it’s just a big white lump and doesn’t at all look stringy. This is because actually DNA itself is very, very small and so you’d need see a single molecule without the help of an extremely powerful microscope that uses electrons instead of light to look at very tiny things. DNA has to be very small to fit inside the cell. In Humans there’s 6ft of DNA in every single cell, and if you were to lay every strand end to end it’d reach to sun which is over 93 million miles away. Then it’d come back again… 600 times!!! That’s a lot of DNA! There are special proteins that wind up the DNA and package it all up so it fits.

Had fun making a mess? Why not send us your comments and pictures! And of course, any questions then drop us a line or comment on the blog.

More Exeriments by Beki

Yeast, friction, pH and goo – all in a day’s work at Einstein’s Kitchen!

 

Despite the torrential rain, the weekend at Green Man was fantastic. Einstein’s Kitchen was a true success, and I’m looking forward to the next time we get to cook up some science!

I had four smaller experiments on the go in the kitchen – blowing up balloons with yeast, lifting up a jar of rice with only a pencil, testing for acids and alkalis using cabbages, and of course the now infamous Oobleck.

So how do you do all of this?  Let’s start at the very beginning…

Can yeast blow up a balloon?

Yeast is a type of micro-organism, so you can only see it under a microscope, but it is a living fungus and commonly used in baking and brewing. In both of these processes the yeast doesn’t breathe like humans do, but survives using fermentation. It uses sugar as a food, and produces CO2 and alcohol. This means that, unlike respiration – where oxygen is taken in and carbon dioxide given out – the yeast produces more and more gas. So, if you trap the yeast in a confined area (like a cola bottle) the extra gas can blow up a balloon!

How do you pick up a jar of rice with only a pencil?

When you first get your jar full of rice all the grains will have lots of gaps between them, full of air. As you push the pencil into the rice, the grains are forced out of the way and will begin to form more ordered arrangements, which reduces the air spaces in the jar. Eventually, the level of rice will drop, as the grains become more and more compacted and closer together – that’s when you top up your jar. As you continue to force the pencil to the bottom of the jar, the friction between the grains of rice, the jar and the pencil increase even more – so much so that eventually (after some perseverance) the force of friction will equal the weight of the jar and the rice. And, hey presto! You’ve lifted the jar!

Using cabbage as a pH indicator.

pH is a measurement of how acidic a solution is – an acid is something with a sour taste, and it has an excess of hydrogen ions (H+). An alkali (also known as a base) has an excess of hydroxide ions (OH-). To learn more about the reactions that happen when you combine the two check out Kay’s blog! Red cabbage has a pigment, or colouring, in it called Flavin. This gives it its purpley-red colour, and is also what can tell you whether something is an acid or alkali. Flavin reacts differently to the amount of H+ ions in a solution, and will turn pink in acids and blue/blue-green in alkalis. You can use it to test out anything you might have at home and even produce a pH scale of your own – showing the range from strong acid, though neutral (the colour doesn’t change), all the way to strong alkali!

 

Oobleck

If you visited us at Green Man Festival I’m sure you won’t have missed the gloopy mixture we had outside… that was Oobleck. Technically speaking Oobleck is a non-Newtonian fluid. This means it doesn’t act like a ‘normal’ fluid, and doesn’t obey the laws that Newton said all fluids should. These are that they would only respond to temperature (freezing water makes a solid; heating up honey makes it more runny) and pressure (squeezing water out of a water pistol moves it faster). Oobleck also responds to shear forces – when you apply sudden and hard force it behaves like a solid, but when you are gentle with it, it acts like a liquid.

So what is going on – how is it that you can punch it, yet your hand will sink into it if you do it slowly? It’s all about the mixture of molecules in the Oobleck – cornflour and water (twice as much cornflour as water). When you are gentle, and use less force (pouring the Oobleck, or sinking your hand into it)  the water molecules can move freely between the cornflour molecules, and they act as a lubricant so it flows like a liquid.  When you apply hard, quick force (by hitting or rolling it) the water molecules can’t fill the gaps, meaning the cornflour molecules are pushed together and friction increases – the Oobleck gets stiffer and acts more like a solid.

What else acts like this? Quicksand for one – that’s why the advice is never to struggle if you’re waist-deep in it – you’ll end up causing it to act like a solid and get even more stuck! Oobleck and quicksand are actually thickening non-Newtonian fluids. Thinning ones are those that become more liquid-y when force is applied – a few examples are paint, toothpaste and ketchup (that’s why tapping the neck of the bottle will release the ketchup!).

It’s not just for fun either – thickening non-Newtonian fluids are also useful. A special kind is being tested in body armour – the way it reacts to sudden force makes it perfect for absorbing the impact of a bullet better than standard vests! It’s also used in the transmissions of some 4×4 vehicles, like Land Rovers, to transfer power between the front and rear wheels when the primary driving wheels start to slip.

And, if you were wondering, the name has been taken from the Dr. Seuss book ‘Bartholomew and the Oobleck’, where King Didd tells his servants he is bored of the same rain, snow, sun and fog and tells them to add some variety to the weather. The result is that sticky green Oobleck falls from the sky and buries everything. It is only when Bartholomew tells him to say “Sorry” that the Oobleck stops falling!

Einstein’s Kitchen was an amazing experience, but it would have been nothing without the budding scientists – both the adults and the kids – who came to our stall and participated in all of our activities. I hope we’ve encouraged everyone to think about how much science there is all around them, and to give some of it a try again at home!

Sweetie Chromatography by Jenna

The Science Brainwaves crew ventured to the Brecon Beacons last week, to take SCIENCE to the unknowing festival goers of Green Man 2010.

Communicating science through the tasty medium of food, the Science Brainwaves team worked up a scientific feast of dry-ice ice cream, DNA extraction, chromatography, fermentation of yeast, non-Newtonian fluids, acid-base reactions, LEDs lit by lemons and our favourite pH indicator red cabbage.

Experiments available in Einstein’s Kitchen at Green Man Festival were:-

  • Dry-ice Ice cream
  • Extraction of DNA from Strawberries
  • Sweetie Chromatography
  • Oobleck (non-Newtonian fluids)
  • A pot of Baking Soda Volcano
  • Coke and Mentos Balloons
  • Acid-Base Indicator (Red Cabbage)
  • Lemon powered lights
  • Picking up a jar of rice with a pencil
  • Yeast Balloons

My main experiment was Sweetie Chromatography, where we separated out the colours of sweets and felt-tips using a technique known as Chromatography.

Chromatography is a method for separating mixtures. The name chromatography comes from the greek chroma- meaning “colour” and graphein- meaning “to write”. Sweetie chromatography used the paper chromatography form of chromatography. The experiment involved the separation of the sweetie colour mixtures by the mobile phase, which in our case was acetone (but other liquids could be used as a solvent such as water, ethanol, nail-varnish remover). The mobile phase was taken up the stationary phase (Whatman filter paper, alternatively coffee filter paper could be used) which separates out the different pigments of colour which make up the sweetie colour.

Visually the aspiring scientists in Einstein’s Kitchen could see the acetone being pulled up the filter paper, bringing with it the colours of the sweets and felt tips.

The take home scientific principle from this experiment is the term molecular solubility. The more soluble [which refers to the how much a substance (in this case the different colours) are capable of being dissolved in a solvent (in this case acetone)] the colour is in the solvent (acetone) the higher up the filter paper it will be deposited. The least soluble colours were deposited at the lowest level on the filter paper.

There were lots of things I enjoyed about the sweetie chromatography but my favourite thing has to be the look on every persons face when they returned to pick up their chromatogram (name given to filter paper once chromatography complete) that look of amazement, surprise and happiness all rolled into one. Proof again that when presented in the right way Science really can be fun.

Stay tuned for further blogs on Einstein’s Kitchen experiments.

Einstein’s Kitchen – Food for Thought!

The Brecon Beacons were the beautiful setting for a music festival called Green Man which happened the weekend of the 20-23rd of August 2010, and the unlikely place to find scientists brewing up fun!

Science Brainwaves, not ones to turn down the chance to get science out to everyone they could, packed up a Transit van and drove the 186 miles to set up a stall called Einstein’s Kitchen in the area of the Green Man festival called Einstien’s Garden (…can you see the link?). Einstein’s Kitchen had a number of different experiments running at various times to get across the basic principles of biology, chemistry and physics to the sodden festival-goers (it may have rained just a wee bit…). All of these experiments were based on things easily found in the kitchen cupboard. Things included using lemons as batteries, Strawberry DNA extractions, sweetie chromatography, dry-ice ice cream, using a cabbage to detect pH, Oobleck, bicarbonate of soda and vinegar volcanos, and more!

If you were lucky enough to attend the festival and had a go at some experiments at our stall then this blog will, for now, be the place to find all the info you need to recreate the experiments in your own kitchen! If you couldn’t get to the festival, then don’t worry! Hopefully you’ll be able to get a feel for the event from our photos and reports. We’ll be sticking up all the information sheets and posters we had there so you can give it all a go yourself! You never know, we may well be taking a new and improved Einstein’s Kitchen to other places… keep your eyes peeled! In the mean time, enjoy!

If you have any comments at all then please, do email/comment/go on our forums!

Lots of science love!

The Science Brainwaves Team

Einstein’s Kitchen

Wow… what a weekend!

It may have rained solidly, but I for one really enjoyed taking science to the revellers at the Green Man festival in Wales!

It was messy, it was informative but most of all it was fun! We even spread the science love (by sticking ‘I heart science’ stickers on to just about everything and everyone)!

 

I’ve just set up a blog to feature all of the Einstein’s Kitchen info – how to carry out the experiments yourself, pictures and reports about the event that all the other Brainwaves team will be contributing to, so go have a look – it will be growing with info as time goes on, but we’re working on giving it it’s own entire section on the website, so keep your eyes peeled.

As it turns out, this may well be the first of many outings that Einstein’s Kitchen makes, and I for one and very excited about it!

 

See you next year, Green Man!

Make Your Own Slime!

 Ok, so it’s no where near Halloween yet, but this stuff is so much fun, so I couldn’t wait.

What you’ll need:

  • Water
  • PVA glue
  • Borax powder (chemical name: sodium tetraborate)
  • Food colouring
  • An old tub and stirring implements

What to do:

Simply mix an equal volume of water to PVA glue (ie if you have 250ml of PVA glue, then you mix it with 250ml water). Add the food colouring and mix thoroughly.

Next, mix half a teaspoon of Borax with about ¾ of the volume of water used before – the borax won’t dissolve in the cold water, but just make sure it’s mixed thoroughly.


Finally, combine the two mixtures together slowly, once it starts forming a glob you can take this out and knead it… and voila! Slime!

 

So how does it work? Basically, PVA glue is made up of a bunch of single units all chained together, and though a thick, or viscous, liquid, these chains are generally able to slide over and past each other with little problem.

The borax (sodium tetraborate) acts to make links between the chains – this results in a thicker substance as all the PVA units are linked together in to a big mesh that doesn’t allow water around the molecules so much. This process is called crosslinking. But is it a solid, or a liquid? The technical term is a non-newtonian liquid. This means that when a force is acted upon it it will act like a solid and resist that force, and the more force you apply, the more solid-like it acts.

 

A Little Adivce

Borax (Sodium Tetraborate) can be an irritant, so please supervise carefully any children whilst doing this and DO NOT eat the glue, borax or final slime! You can handle the slime, but be sure to wash it off, and it should come off in warm, soapy water if you get it on your clothes. The slime can be stored in a sealed container for a couple of weeks int the fridge.

 

 

If you make some of your own slime, why not send us some photos and how you’ve used it to ask@sciencebrainwaves.com